CLIPPER
An add-on to the Trans-Proteomic Pipeline for the automated analysis of TAILS N-terminomics data
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Author / Creator
Date
2012-12
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Data analysis in proteomics is complex and with the extra challenges involved in the interpretation of data from N-terminomics experiments, this can be daunting. Therefore, we have devised a rational pipeline of steps to approach N-terminomics data analysis in a statistically-based and valid manner. We have automated these steps in CLIPPER, an add-on to the Trans-Proteomic Pipeline (TPP). Applying CLIPPER to the analysis of N-terminomics data generated by terminal amine isotopic labeling of substrates (TAILS) enables high confidence peptide to protein assignment, protein N-terminal characterization and annotation, and for protease analysis readily allows protease substrate discovery with high confidence.
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published
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Journal / series
Volume
393 (12)
Pages / Article No.
1477 - 1483
Publisher
De Gruyter
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Edition / version
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Subject
Degradomics; N-terminomics; Protease; Proteomics; Terminal amine isotopic labeling of substrates (TAILS); Trans-Proteomic Pipeline (TPP)
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Notes
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher