VEGF profiling and angiogenesis in human microtissues
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Date
2005-08
Publication Type
Journal Article
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yes
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Abstract
Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in microtissues by gravity-enforced self-aggregation in hanging drops we (i) studied size and age-dependent VEGF production of microtissues in comparison to isogenic monolayer cultures, (ii) characterized the self-organization and VEGF-production potential of mixed-cell spheroids, (iii) analyzed VEGF-dependent capillary formation of human umbilical vein endothelial cells (HUVECs) cells coated onto several human primary cell spheroids, and (iv) profiled endostatin action on vascularization in human microtissues. Precise understanding of vascularization in human microtissues may foster advances in clinical tissue implant engineering, tumor treatment, as well as drug discovery and drug-function analysis. © 2005 Elsevier B.V. All rights reserved.
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published
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Journal / series
Volume
118 (2)
Pages / Article No.
213 - 229
Publisher
Elsevier
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Edition / version
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Date collected
Date created
Subject
Tissue engineering; Cell-based therapies; Self-assembly; Vascularization
Organisational unit
03694 - Fussenegger, Martin / Fussenegger, Martin
Notes
Received 26 September 2004, Revised 25 February 2005, Accepted 04 March 2005, Available online 13 June 2005.