The Plant Journal

Journal: The Plant Journal

Abbreviation

Plant j.

Publisher

Wiley-Blackwell

ISSN

0960-7412
1365-313X

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Publications 1 - 10 of 58

Recycling of pyridoxine (vitamin B6) by PUP1 in Arabidopsis
Szydlowski, Nicolas; Bürkle, Lukas; Pourcel, Lucille; et al. (2013)
The Plant Journal
Tomato fruit carotenoid biosynthesis is adjusted to actual ripening progression by a light-dependent mechanism
Llorente, Briardo; D'Andrea, Lucio; Aguila Ruiz-Sola, M.; et al. (2016)
The Plant Journal
ABC1K1/PGR6 kinase
Martinis, J.; Glauser, G.; Valimareanu, S.; et al. (2014)
The Plant Journal
Mutagenesis of cysteine 81 prevents dimerization of the APS1 subunit of ADP-glucose pyrophosphorylase and alters diurnal starch turnover in Arabidopsis thaliana leaves
Hadrich, Nadja; Hendriks, Janneke H.M.; Kötting, Oliver; et al. (2012)
The Plant Journal
The Arabidopsis thaliana FPP synthase isozymes have overlapping and specific functions in isoprenoid biosynthesis, and complete loss of FPP synthase activity causes early developmental arrest
Closa, Marta; Vranová, Eva; Bortolotti, Cristina; et al. (2010)
The Plant Journal
Enzymatic, but not non-enzymatic, O-1(2)-mediated peroxidation of polyunsaturated fatty acids forms part of the EXECUTER1-dependent stress response program in the flu mutant of Arabidopsis thaliana
Przybyla, Dominika; Goebel, Cornelia; Imboden, Andre; et al. (2008)
The Plant Journal
Concurrent activation of cell death-regulating signaling pathways by singlet oxygen in Arabidopsis thaliana
Danon, Antoine; Miersch, Otto; Felix, Georg; et al. (2005)
The Plant Journal
A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis
Schramm, Franziska; Larkindale, Jane; Kiehlmann, Elke; et al. (2008)
The Plant Journal
H3K23me1 is an evolutionarily conserved histone modification associated with CG DNA methylation in Arabidopsis
Trejo-Arellano, Minerva S.; Mahrez, Walid; Nakamura, Miyuki; et al. (2017)
The Plant Journal
A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19
Jay, Florence; Brioudes, Florian; Voinnet, Olivier (2023)
The Plant Journal
Transient transgenic expression accelerates pharming and facilitates protein studies in plants. One embodiment of the approach involves leaf infiltration of Agrobacterium strains whose T-DNA is engineered with the gene(s) of interest. However, gene expression during 'agro-infiltration' is intrinsically and universally impeded by the onset of post-transcriptional gene silencing (PTGS). Nearly 20 years ago, a simple method was developed, whereby co-expression of the tombusvirus-encoded P19 protein suppresses PTGS and thus enhances transient gene expression. Yet, how PTGS is activated and suppressed by P19 during the process has remained unclear to date. Here, we address these intertwined questions in a manner also rationalizing how vastly increased protein yields are achieved using a minimal viral replicon as a transient gene expression vector. We also explore, in side-by-side analyses, why some proteins do not accumulate to the expected high levels in the assay, despite vastly increased mRNA levels. We validate that enhanced co-expression of multiple constructs is achieved within the same transformed cells, and illustrate how the P19 system allows rapid protein purification for optimized downstream in vitro applications. Finally, we assess the suitability of the P19 system for subcellular localization studies - an originally unanticipated, yet increasingly popular application - and uncover shortcomings of this specific implement. In revisiting the P19 system using contemporary knowledge, this study sheds light onto its hitherto poorly understood mechanisms while further illustrating its versatility but also some of its limits.

Publications 1 - 10 of 58

Journal: The Plant Journal

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