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Optimization of Critical Hairpin Features Allows miRNA-based Gene Knockdown Upon Single-copy Transduction
(2014)Molecular Therapy - Nucleic AcidsGene knockdown using micro RNA (miRNA)-based vector constructs is likely to become a prominent gene therapy approach. It was the aim of this study to improve the efficiency of gene knockdown through optimizing the structure of miRNA mimics. Knockdown of two target genes was analyzed: CCR5 and green fluorescent protein. We describe here a novel and optimized miRNA mimic design called mirGE comprising a lower stem length of 13 base pairs ...Journal Article -
A way forward for Cosmic Shear: Monte-Carlo Control Loops
(2014)Physics of the Dark UniverseWeak lensing by large scale structure or ‘cosmic shear’ is a potentially powerful cosmological probe to shed new light on Dark Matter, Dark Energy and Modified Gravity. It is based on the weak distortions induced by large-scale structures on the observed shapes of distant galaxies through gravitational lensing. While the potentials of this purely gravitational effect are great, results from this technique have been hampered because the ...Journal Article -
Antihyperalgesia by α2-GABAA Receptors Occurs Via a Genuine Spinal Action and Does Not Involve Supraspinal Sites
(2014)NeuropsychopharmacologyJournal Article -
Developing a GIS-Based Visual-Acoustic 3D Simulation for Wind Farm Assessment
(2014)ISPRS International Journal of Geo-InformationPublic landscape impact assessment of renewable energy installations is crucial for their acceptance. Thus, a sound assessment basis is crucial in the implementation process. For valuing landscape perception, the visual sense is the dominant human sensory component. However, the visual sense provides only partial information about our environment. Especially when it comes to wind farm assessments, noise produced by the rotating turbine ...Journal Article -
The Intestinal Microbiota Interferes with the microRNA Response upon Oral Listeria Infection
(2013)mBioThe intestinal tract is the largest reservoir of microbes in the human body. The intestinal microbiota is thought to be able to modulate alterations of the gut induced by enteropathogens, thereby maintaining homeostasis. Listeria monocytogenes is the agent of listeriosis, an infection transmitted to humans upon ingestion of contaminated food. Crossing of the intestinal barrier is a critical step of the infection before dissemination ...Journal Article -
SATB1 collaborates with loss of p16 in cellular transformation
(2013)OncogeneTumor progression is associated with invasiveness and metastatic potential. The special AT-rich binding protein 1 (SATB1) has been identified as a key factor in the progression of breast cancer cells to a malignant phenotype and is associated with progression of human tumors. In normal development, SATB1 coordinates gene expression of progenitor cells by functioning as a genome organizer. In contrast to progenitor and tumor cells, SATB1 ...Journal Article -
Mechanisms of haptoglobin protection against hemoglobin peroxidation triggered endothelial damage
(2013)Cell Death and DifferentiationJournal Article -
Frequent floods in the European Alps coincide with cooler periods of the past 2500 years
(2013)Scientific ReportsSevere floods triggered by intense precipitation are among the most destructive natural hazards in Alpine environments, frequently causing large financial and societal damage. Potential enhanced flood occurrence due to global climate change would thus increase threat to settlements, infrastructure, and human lives in the affected regions. Yet, projections of intense precipitation exhibit major uncertainties and robust reconstructions of ...Journal Article -
Prediction Stability in a data-based mechanistic model of σF during sporulation in Bacillus subtilis
(2013)Scientific ReportsMathematical modeling of biological networks can help to integrate a large body of information into a consistent framework, which can then be used to arrive at novel mechanistic insight and predictions. We have previously developed a detailed, mechanistic model for the regulation of σ F during sporulation in Bacillus subtilis. The model was based on a wide range of quantitative data, and once fitted to the data, the model made predictions ...Journal Article -
Pharmacologically Controlled Protein Switch for ON-OFF Regulation of Growth Factor Activity
(2013)Scientific ReportsThe precise manipulation of growth factor signaling is central to the progress of tissue engineering. Methods for direct time-resolved activation of signaling pathways through controlled receptor dimerization have been reported; however, these suffer from the risks associated with gene transfer. Here we present an alternative gene transfer-free approach in the form of a protein switch featuring pharmacologically controlled ON-OFF regulation ...Journal Article