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Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium
(2017)Molecular Biology of the CellYeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and ...Journal Article -
Temporal differences in plant growth and root exudation of two Brachiaria grasses in response to low phosphorus supply
(2017)Tropical Grasslands - Forrajes TropicalesExploiting the natural variability of Brachiaria forage germplasm to identify forage grasses adapted to infertile acid soils that contain very low available phosphorus (P) is an important research objective for improving livestock production in the tropics. The objective of this study was to determine the differences in the release of root biochemical markers, i.e. carboxylates and acid phosphatases (APases), during the development of P ...Journal Article -
Local Arp2/3 dependent actin assembly modulates applied traction force during apCAM adhesion site maturation
(2017)Molecular Biology of the CellHomophilic binding of immunoglobulin superfamily molecules such as the Aplysia cell adhesion molecule (apCAM) leads to actin filament assembly near nascent adhesion sites. Such actin assembly can generate significant localized forces that have not been characterized in the larger context of axon growth and guidance. We used apCAM-coated bead substrates applied to the surface of neuronal growth cones to characterize the development of ...Journal Article