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Spatiotemporal proteomics uncovers cathepsin-dependent host cell death during bacterial infection
(2018)bioRxivImmune cells need to swiftly and effectively respond to invading pathogens. This response relies heavily on rapid protein synthesis and accurate cellular targeting to ensure pathogen destruction. In return, pathogens intercept this response to ensure their survival and proliferation. To gain insight into this dynamic interface, we combined click-chemistry with pulsed stable isotope labeling of amino acids (pSILAC-AHA) in cell culture to ...Working Paper -
Cardiovascular responses to antigravity muscle loading during head-down tilt at rest and after dynamic exerrcises
(2018)bioRxivThe physiological processes underlying hemodynamic homeostasis can be modulated by muscle activity and gravitational loading. The effects of antigravity muscle activity on cardiovascular regulation has been observed during orthostatic stress. Here, we evaluated such effects during head-down tilt (HDT). In this posture, the gravitational gradient along the body is different than in upright position, leading to increased central blood volume ...Working Paper -
Multi-platform discovery of haplotype-resolved structural variation in human genomes
(2018)bioRxivThe incomplete identification of structural variants from whole-genome sequencing data limits studies of human genetic diversity and disease association. Here, we apply a suite of long- and short-read, strand-specific sequencing technologies, optical mapping, and variant discovery algorithms to comprehensively analyze three human parent-child trios to define the full spectrum of human genetic variation in a haplotype-resolved manner. We ...Working Paper