Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
dc.contributor.author
Vörös, Janos
dc.contributor.author
Grieshaber, Dorothee
dc.contributor.author
De Lange, Victoria
dc.contributor.author
Hirt, Thomas
dc.contributor.author
Lu, Zhihua
dc.date.accessioned
2019-06-14T13:13:29Z
dc.date.available
2017-06-08T20:24:25Z
dc.date.available
2019-06-14T13:13:29Z
dc.date.issued
2008-12
dc.identifier.issn
1424-8220
dc.identifier.other
10.3390/s8127894
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/11810
dc.identifier.doi
10.3929/ethz-b-000011810
dc.description.abstract
The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
High sensitivity
en_US
dc.subject
antigen detection
en_US
dc.subject
biosensor
en_US
dc.subject
lipid vesicles
en_US
dc.subject
quartz crystal microbalance
en_US
dc.title
Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Sensors
ethz.journal.volume
8
en_US
ethz.journal.issue
12
en_US
ethz.pages.start
7894
en_US
ethz.pages.end
7903
en_US
ethz.version.deposit
publishedVersion
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ethz.publication.place
Basel
ethz.publication.status
published
en_US
ethz.leitzahl
03656 - Bösiger, Peter
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::03741 - Vörös, Janos / Vörös, Janos
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::03741 - Vörös, Janos / Vörös, Janos
ethz.leitzahl.certified
03656 - Bösiger, Peter
ethz.date.deposited
2017-06-08T20:24:42Z
ethz.source
ECIT
ethz.identifier.importid
imp59364c0b5a70a71643
ethz.ecitpid
pub:23036
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T18:56:09Z
ethz.rosetta.lastUpdated
2019-06-14T13:13:37Z
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true
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