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Date
2016Type
- Book Chapter
ETH Bibliography
yes
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Abstract
Owing to their modular and highly specific DNA recognition mode, transcription activator-like effector nucleases (TALENs) have been rapidly adopted by the scientific community for the purpose of generating site-specific double-strand breaks (DSBs) on a DNA molecule. A pair of TALENs can be used to produce random insertions or deletions of various lengths via nonhomologous end-joining or together with a homologous donor DNA to induce precise sequence alterations by homologous recombination (HR). Here, we describe a method for TALEN assembly (easyT) and a strategy for genome engineering via HR. Show more
Publication status
publishedBook title
Polycomb Group ProteinsJournal / series
Methods in Molecular BiologyVolume
Pages / Article No.
Publisher
Humana PressSubject
TALEN; Genome engineering; Site-specific gene integrationOrganisational unit
03748 - Paro, Renato (emeritus) / Paro, Renato (emeritus)
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ETH Bibliography
yes
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