The capillary gap sampler, a new microfluidic platform for direct coupling of automated solid-phase microextraction with ESI-MS
Open access
Date
2017-11Type
- Journal Article
Abstract
A new technology for rapid, automated coupling of solid-phase microextraction and mass spectrometry is introduced. Use of a so-called capillary gap sampler for automated solid-phase microextraction and direct delivery of the extracted analytes to a mass spectrometer provides certain advantages over existing technologies: coupling of the capillary gap sampler to a mass spectrometer offers quick, automated, and site-specific extraction from very low volume samples. High stability, reusability, and repeatability were achieved through systematic optimization. Diazepam, oxazepam, and nordiazepam were used as test compounds in all experiments. The ability of the sampler to extract benzodiazepines from human plasma (limit of detection 0.3 μg/mL) in the therapeutic range was confirmed. A linear dynamic range from 1 to 1000 ng/mL for all three analytes was found. The relative standard deviation of 20 extractions was between 11% and 17%, for oxazepam, nordiazepam, and diazepam, indicating acceptable repeatability of the method. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000201949Publication status
publishedExternal links
Journal / series
Analytical and Bioanalytical ChemistryVolume
Pages / Article No.
Publisher
SpringerSubject
Capillary gap sampler; solid-phase microextraction; Benzodiazepines; Human plasma; Electrospray ionization mass spectrometryOrganisational unit
03430 - Zenobi, Renato / Zenobi, Renato
Funding
159929 - Soft ionization mass spectrometry for studying noncovalent interactions (SNF)
Notes
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.More
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