Extracerebral Tissue Damage in the Intraluminal Filament Mouse Model of Middle Cerebral Artery Occlusion
Open access
Date
2017-03-13Type
- Journal Article
Abstract
Middle cerebral artery occlusion is the most common model of focal cerebral ischemia in the mouse. In the surgical procedure, the external carotid artery (ECA) is ligated; however, its effect on the tissue supplied by the vessel has not been described so far. C57BL/6 mice underwent 1 h of transient MCAO (tMCAO) or sham surgery. Multi-spectral optoacoustic tomography was employed at 30 min after surgery to assess oxygenation in the temporal muscles. Microstructural changes were assessed with magnetic resonance imaging and histological examination at 24 h and 48 h after surgery. Ligation of the ECA resulted in decreased oxygenation of the left temporal muscle in most sham-operated and tMCAO animals. Susceptible mice of both groups exhibited increased T2 relaxation times in the affected muscle with histological evidence of myofibre degeneration, interstitial edema, and neutrophil influx. Ligatures had induced an extensive neutrophil-dominated inflammatory response. ECA ligation leads to distinct hypoxic degenerative changes in the tissue of the ECA territory and to ligature-induced inflammatory processes. An impact on outcome needs to be considered in this stroke model. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000237483Publication status
publishedExternal links
Journal / series
Frontiers in NeurologyVolume
Pages / Article No.
Publisher
Frontiers MediaSubject
middle cerebral artery occlusion; mouse; ischemia; external carotid artery; muscle degenerationOrganisational unit
03750 - Rudin, Markus (emeritus)
Funding
136822 - Molecular imaging of neutrophil protease-mediated blood-brain barrier impairment and thrombosis after cerebral ischemia (SNF)
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