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High-spin Metal Centres in Dipolar EPR Spectroscopy
(2018)ChimiaThe substitution of Mg2+ by Mn2+ in the bacterial DnaB helicase from Helicobacter pylori, an ATP:Mg2+-fuelled protein engine, allows electron paramagnetic resonance (EPR) spectroscopy to be performed on this system. EPR experiments make it possible to monitor nucleotide binding and to estimate the fraction of bound Mn2+ through relaxation measurements. Furthermore, by measuring spin–spin distances we probe the geometry within such multimeric ...Journal Article -