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A universal method for the rapid isolation of all known classes of functional silencing small RNAs
(2020)Nucleic Acids ResearchDiverse classes of silencing small (s)RNAs operate via ARGONAUTE-family proteins within RNA-induced-silencing-complexes (RISCs). Here, we have streamlined various embodiments of a Q-sepharose-based RISC-purification method that relies on conserved biochemical properties of all ARGONAUTEs. We show, in multiple benchmarking assays, that the resulting 15-min benchtop extraction procedure allows simultaneous purification of all known classes ...Journal Article -
Eukaryotic life without tQ(CUG): the role of Elongator-dependent tRNA modifications in Dictyostelium discoideum
(2020)Nucleic Acids ResearchIn the Elongator-dependent modification pathway, chemical modifications are introduced at the wobble uridines at position 34 in transfer RNAs (tRNAs), which serve to optimize codon translation rates. Here, we show that this three-step modification pathway exists in Dictyostelium discoideum, model of the evolutionary superfamily Amoebozoa. Not only are previously established modifications observable by mass spectrometry in strains with the ...Journal Article -
High-precision stable isotope analysis of <5 μg CaCO3 samples by continuous-flow mass spectrometry
(2020)Rapid Communications in Mass Spectrometry© 2020 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd Rationale: Oxygen (δ18O) and carbon (δ13C) isotope analysis of foraminifera and other CaCO3 samples has been a key technique for paleoceanographical and paleoclimatological research for more than 60 years. There is ongoing demand for the analysis of ever smaller CaCO3 samples, driven, for example, by the desire to analyse single specimen ...Journal Article -
Liquid repellency enhancement through flexible microstructures
(2020)Science AdvancesArtificial liquid-repellent surfaces have attracted substantial scientific and industrial attention with a focus on creating functional topological features; however, the role of the underlying structures has been overlooked. Recent developments in micro-nanofabrication allow us now to construct a skin-muscle type system combining interfacial liquid repellence atop a mechanically functional structure. Specifically, we design surfaces ...Journal Article -
CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen
(2020)The Plant JournalCellulose microfibrils synthesized by CELLULOSE SYNTHASE COMPLEXES (CSCs) are the main load-bearing polymers in wood. CELLULOSE SYNTHASE INTERACTING1 (CSI1) connects CSCs with cortical microtubules, which align with cellulose microfibrils. Mechanical properties of wood are dependent on cellulose microfibril alignment and structure in the cell walls, but the molecular mechanism(s) defining these features is unknown. Herein, we investigated ...Journal Article -
The Molecule of the Week: A Didactic Tool for Teaching General Chemistry
(2020)ChimiaThe molecule of the week is regularly used by the author in his first-semester course General Chemistry I (Inorganic Chemistry) to illustrate, exemplify, and deepen fundamental aspects and concepts treated in the course. Pure sulfuric acid and its autoprotolysis is used to introduce the concepts of protochemical window and superacidity. The drug Auranofin serves at showing fundamental aspects of gold redox and coordination chemistry and ...Journal Article -
Terrestrial Biomolecular Burial Efficiencies on Continental Margins
(2020)Journal of Geophysical Research: BiogeosciencesThe Authors. The fate of terrestrial organic carbon (OCterr) exported from large rivers in marginal seas is an integral component of land-ocean-atmosphere carbon dynamics and influences on atmospheric CO2 concentrations on millennial and longer timescales. In this study, we employ a novel approach to constrain burial efficiencies for source-specific terrestrial biomolecules (long-chain n-alkanes and n-fatty acids) in two river-marginal ...Journal Article -
Is deflation costly after all? The perils of erroneous historical classifications
(2020)Journal of Applied EconometricsI estimate average economic activity during periods of inflation and deflation while accounting for measurement errors in 19th century prices. These measurement errors lead to underestimation (overestimation) of economic activity during periods of inflation (deflation). By exploiting multiple deflation indicators, it is possible to recover the true relationship; the shortfall of US industrial production growth during periods of deflation ...Journal Article -
Magnetic fields modulate metabolism and gut microbiome in correlation withPgc-1 alpha expression: Follow-up to an in vitro magnetic mitohormetic study
(2020)The FASEB JournalExercise modulates metabolism and the gut microbiome. Brief exposure to low mT-range pulsing electromagnetic fields (PEMFs) was previously shown to accentuate in vitro myogenesis and mitochondriogenesis by activating a calcium-mitochondrial axis upstream of PGC-1 alpha transcriptional upregulation, recapitulating a genetic response implicated in exercise-induced metabolic adaptations. We compared the effects of analogous PEMF exposure ...Journal Article -
In vitro 3D phenotypic drug screen identifies celastrol as an effective in vivo inhibitor of polycystic kidney disease
(2020)Journal of molecular cell biologyPolycystic kidney disease (PKD) is a prevalent genetic disorder, characterized by the formation of kidney cysts that progressively lead to kidney failure. The currently available drug tolvaptan is not well tolerated by all patients and there remains a strong need for alternative treatments. The signaling rewiring in PKD that drives cyst formation is highly complex and not fully understood. As a consequence, the effects of drugs are sometimes ...Journal Article