Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels
Abstract
Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Brazilian cassava cultivars and report the modification of the protocol for the genetic transformation of Verdinha (BRS 222), a recalcitrant cultivar with high potential for protein production that is extensively used by farmers in Brazil. Show more
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https://doi.org/10.3929/ethz-b-000357942Publication status
publishedExternal links
Journal / series
Methods and ProtocolsVolume
Pages / Article No.
Publisher
MDPISubject
Cassava; Somatic embryo; FEC; Transformation; Agrobacterium; Farmer-preferred cultivarOrganisational unit
02030 - Dep. Biologie / Dep. of Biology
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