Use of bifidobacterial specific terminal restriction fragment length polymorphisms to complement next generation sequence profiling of infant gut communities
dc.contributor.author
Lewis, Zachery T.
dc.contributor.author
Bokulich, Nicholas
dc.contributor.author
Kalanetra, Karen M.
dc.contributor.author
Ruiz-Moyano, Santiago
dc.contributor.author
Underwood, Mark A.
dc.contributor.author
Mills, David A.
dc.date.accessioned
2020-08-17T08:41:05Z
dc.date.available
2020-08-12T10:15:31Z
dc.date.available
2020-08-17T08:41:05Z
dc.date.issued
2013-02
dc.identifier.other
10.1016/j.anaerobe.2012.12.005
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/431202
dc.description.abstract
Bifidobacteria are intestinal anaerobes often associated with gut health. Specific bifidobacterial species are particularly common in the gastrointestinal tract of breast-fed infants. Current short read next-generation sequencing approaches to profile fecal microbial ecologies do not discriminate bifidobacteria to the species level. Here we describe a low-cost terminal restriction fragment length polymorphism (TRFLP) procedure to distinguish between the common infant-associated bifidobacterial species. An empirical database of TRF sizes was created from both common reference strains and well-identified isolates from infant feces. Species-specific quantitative PCR validated bifidobacterial-specific TRFLP profiles from infant feces. These results indicate that bifidobacterial-specific TRFLP is a useful method to monitor intestinal bifidobacterial populations from infant fecal samples. When used alongside next generation sequencing methods that detect broader population levels at lower resolution, this high-throughput, low-cost tool can help clarify the role of bifidobacteria in health and disease.
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.subject
TRFLP
en_US
dc.subject
Bifidobacteria
en_US
dc.subject
Infants
en_US
dc.subject
Gut microbiota
en_US
dc.subject
NGS
en_US
dc.title
Use of bifidobacterial specific terminal restriction fragment length polymorphisms to complement next generation sequence profiling of infant gut communities
en_US
dc.type
Journal Article
dc.date.published
2012-12-20
ethz.journal.title
Anaerobe
ethz.journal.volume
19
en_US
ethz.pages.start
62
en_US
ethz.pages.end
69
en_US
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09714 - Bokulich, Nicholas / Bokulich, Nicholas
en_US
ethz.date.deposited
2020-08-12T10:15:39Z
ethz.source
BATCH
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-08-17T08:41:13Z
ethz.rosetta.lastUpdated
2022-03-29T02:55:38Z
ethz.rosetta.versionExported
true
ethz.COinS
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Journal Article [128937]