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dc.contributor.author
Mayr, Magdalena J.
dc.contributor.author
Zimmermann, Matthias
dc.contributor.author
Dey, Jason
dc.contributor.author
Wehrli, Bernhard
dc.contributor.author
Bürgmann, Helmut
dc.date.accessioned
2020-09-21T13:21:02Z
dc.date.available
2020-09-19T02:58:13Z
dc.date.available
2020-09-21T13:21:02Z
dc.date.issued
2020
dc.identifier.issn
1726-4170
dc.identifier.issn
1726-4170
dc.identifier.other
10.5194/bg-17-4247-2020
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/441306
dc.identifier.doi
10.3929/ethz-b-000441306
dc.description.abstract
In lakes, large amounts of methane are produced in anoxic sediments. Methane-oxidizing bacteria effectively convert this potent greenhouse gas into biomass and carbon dioxide. These bacteria are present throughout the water column, where methane concentrations can range from nanomolar to millimolar. In this study, we tested the hypothesis that methanotroph assemblages in a seasonally stratified freshwater lake are adapted to the contrasting methane concentrations in the epi- and hypolimnion. We further hypothesized that lake overturn would change the apparent methane oxidation kinetics as more methane becomes available in the epilimnion. In addition to the change in the methane oxidation kinetics, we investigated changes in the transcription of genes encoding methane monooxygenase, the enzyme responsible for the first step of methane oxidation, with metatranscriptomics. Using laboratory incubations of the natural microbial communities, we show that the half-saturation constant (Km) for methane – the methane concentration at which half the maximum methane oxidation rate is reached – was 20 times higher in the hypolimnion than in the epilimnion during stable stratification. During lake overturn, however, the kinetic constants in the epi- and hypolimnion converged along with a change in the transcriptionally active methanotroph assemblage. Conventional particulate methane monooxygenase appeared to be responsible for methane oxidation under different methane concentrations. Our results suggest that methane availability is one important factor for creating niches for methanotroph assemblages with well-adapted methane oxidation kinetics. This rapid selection and succession of adapted lacustrine methanotroph assemblages allowed the previously reported high removal efficiency of methane transported to the epilimnion to be maintained – even under rapidly changing conditions during lake overturn. Consequently, only a small fraction of methane stored in the anoxic hypolimnion is emitted to the atmosphere.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Copernicus
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Lake mixing regime selects apparent methane oxidation kinetics of the methanotroph assemblage
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-04-26
ethz.journal.title
Biogeosciences
ethz.journal.volume
17
en_US
ethz.journal.issue
16
en_US
ethz.pages.start
4247
en_US
ethz.pages.end
4259
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Göttingen
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-09-19T02:58:19Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-09-21T13:21:18Z
ethz.rosetta.lastUpdated
2020-09-21T13:21:18Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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