Pulsed electric field treatment enhances lipid bioaccessibility while preserving oxidative stability in Chlorella vulgaris
Abstract
There is growing demand for gentle technologies to improve the lipid bioaccessibility (BA) of Chlorella vulgaris biomass while preserving cell integrity and therefore oxidative stability. Pulsed electric field treatment (PEF, 5 μs at 20 kV cm−1, 31.8 kJ kg−1sus) led to an enhancement in lipid BA from 4–7.8% (untreated) to 18.7–20.9%. To reach such a level of BA, incubation in buffer after the treatment (12 h at 25/37 °C, 48 h at 4 °C) was required. As hypothesized, PEF preserved cell integrity, as shown by particle size and scanning electron microscopy analyses, as well as oxidative stability of the biomass over 3 months at 40 °C. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future work should focus on further understanding the mechanism behind incubation after PEF and studying the potential effect played by endogenous cell wall-degrading enzymes. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000520628Publication status
publishedExternal links
Journal / series
Innovative Food Science & Emerging TechnologiesVolume
Pages / Article No.
Publisher
ElsevierSubject
Microalgae; Pulsed electric field; Bioaccessibility; Lipids; EnzymesOrganisational unit
09571 - Mathys, Alexander / Mathys, Alexander
02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
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