Analysis of Salmonella Persister Population Sizes, Dynamics of Gut Luminal Seeding, and Plasmid Transfer in Mouse Models of Salmonellosis
Metadata only
Date
2021-09-30Type
- Book Chapter
ETH Bibliography
yes
Altmetrics
Abstract
A previously unappreciated link between persisters and the emergence and spread of antibiotic resistance has been recently established. The bulk of this research has been conducted in vitro, but some studies are beginning to elucidate the importance of persister reservoirs in both antibiotic treatment failure and the spread of antibiotic resistance using in vivo models. In order to further this research, careful analyses of the mechanisms of persister reservoir formation as well as the dynamics of persister survival and postantibiotic regrowth are of importance. Here, we present a mouse model to quantitatively study Salmonella persisters in vivo. By using neutral unique sequence barcodes, we describe the quantitative analysis of rare events (aka bottlenecks) associated with persister reservoir formation, survival, and reseeding of the gut lumen. This provides quantitative data for persister-fueled plasmid transfer in vivo. Although this chapter describes analysis of Salmonella persisters in a mouse model, these concepts can be applied to any experimental system provided that tractable experimental systems are present. Show more
Publication status
publishedBook title
Bacterial PersistenceJournal / series
Methods in Molecular BiologyVolume
Pages / Article No.
Publisher
HumanaSubject
Persistence; Mouse model; Salmonella Typhimurium; Ceftriaxone; Plasmid transfer; Antibiotic resistance plasmids; ESBL; Pathogen evolutionOrganisational unit
03589 - Hardt, Wolf-Dietrich / Hardt, Wolf-Dietrich
Funding
192567 - Mechanisms controlling the Salmonella Typhimurium gut infection (SNF)
167121 - Towards quantification of the contribution of plasmids to the spread of antibiotic resistance (SNF)
More
Show all metadata
ETH Bibliography
yes
Altmetrics