Open access
Date
2022-03-21Type
- Review Article
Abstract
Recent advances in methods for single-cell analyses and barcoding strategies have led to considerable progress in research. The development of multiplexed assays offers the possibility to conduct parallel analyses of multiple factors and processes for comprehensive characterization of cellular and molecular states in health and disease. These technologies have expanded extremely rapidly in the past years and constantly evolve and provide better specificity, precision and resolution. This review summarizes recent progress in single-cell multiomics approaches, and focuses, in particular, on the most innovative techniques that integrate genome, epigenome and transcriptome profiling. It describes the methodologies, discusses their advantages and limitations, and explains how they have been applied to studies on cell heterogeneity and differentiation, and epigenetic reprogramming. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000542587Publication status
publishedExternal links
Journal / series
Frontiers in Cell and Developmental BiologyVolume
Pages / Article No.
Publisher
Frontiers MediaSubject
single-cell; multiomics; genomics; epigenomics; transcriptomics; chromatin accessibilityOrganisational unit
03518 - Mansuy, Isabelle / Mansuy, Isabelle
Funding
182880 - NCCR RNA&Disease (51NF40-182880): Flexibility Grant (SNF)
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