A molecular framework for proximal secondary vein branching in the Arabidopsis thaliana embryo
Abstract
Over time, plants have evolved flexible self-organizing patterning mechanisms to adapt tissue functionality to continuous organ growth. An example of this process is the multicellular organization of cells into a vascular network in foliar organs. An important, yet poorly understood component of this process is secondary vein branching; a mechanism employed to extend vascular tissues throughout cotyledons’ surface. Here, we uncovered two distinct branching mechanisms during embryogenesis by analysing the discontinuous vein network of cotyledon vascular pattern 2 (cvp2) cvp2-like 1 (cvl1). Similar to wild type, distal veins in cvp2 cvl1 embryos arise from the bifurcation of cell files contained in the midvein, whereas proximal branching is absent in this mutant. Restoration of this process can be achieved by increasing OCTOPUS dosage as well as by silencing RECEPTOR LIKE PROTEIN KINASE 2 (RPK2) expression. While RPK2-dependent rescue of cvp2 cvl1 is auxin- and CLE peptide independent, distal branching involves auxin polar transport and follows a distinct regulatory mechanism. Our work defines a genetic network conferring plasticity to Arabidopsis embryos to adapt the spatial configuration of vascular tissues to organ growth. Show more
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https://doi.org/10.3929/ethz-b-000553423Publication status
publishedExternal links
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Company of BiologistsSubject
Auxin canalization; Cell division; CLE peptides; Developmental plasticity; Embryogenesis; Vein patterningOrganisational unit
09493 - Rodriguez-Villalon, Antia (ehemalig) / Rodriguez-Villalon, Antia (former)
Funding
160201 - Phosphoinositides- Molecular analysis of new lipid regulators in plant phloem differentiation (SNF)
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Is new version of: https://doi.org/10.3929/ethz-b-000510653
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