Open access
Date
2022Type
- Journal Article
ETH Bibliography
yes
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Abstract
Environmental and host-associated microbiomes are typically diverse assemblages of organisms performing myriad activities and
engaging in a network of interactions that play out in spatially structured contexts. As the sum of these activities and interactions
give rise to overall microbiome function, with important consequences for environmental processes and human health, elucidating
specific microbial activities within complex communities is a pressing challenge. Single-cell stable isotope probing (SC-SIP)
encompasses multiple techniques that typically utilize Raman microspectroscopy or nanoscale secondary ion mass spectrometry
(NanoSIMS) to enable spatially resolved tracking of isotope tracers in cells, cellular components, and metabolites. SC-SIP techniques
are uniquely suited for illuminating single-cell activities in microbial communities and for testing hypotheses about cellular
functions generated for example from meta-omics datasets. Here, we illustrate the insights enabled by SC-SIP techniques by
reviewing selected applications in microbiology and offer a perspective on their potential for future research. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000585170Publication status
publishedExternal links
Journal / series
ISME CommunicationsVolume
Pages / Article No.
Publisher
SpringerOrganisational unit
09467 - Stocker, Roman / Stocker, Roman
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ETH Bibliography
yes
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