Abstract
Light-based vat-polymerization bioprinting enables computer-aided patterning of 3D cell-laden structures in a point-by-point, layer-by-layer or volumetric manner, using vat (vats) filled with photoactivatable bioresin (bioresins). This collection of technologies — divided by their modes of operation into stereolithography, digital light processing and volumetric additive manufacturing — has been extensively developed over the past few decades, leading to broad applications in biomedicine. In this Primer, we illustrate the methodology of light-based vat-polymerization 3D bioprinting from the perspectives of hardware, software and bioresin selections. We follow with discussions on methodological variations of these technologies, including their latest advancements, as well as elaborating on key assessments utilized towards ensuring qualities of the bioprinting procedures and products. We conclude by providing insights into future directions of light-based vat-polymerization methods. Show more
Publication status
publishedExternal links
Journal / series
Nature Reviews Methods PrimersVolume
Pages / Article No.
Publisher
NatureSubject
Biomaterials; Biomedical engineering; Mechanical engineering; Regenerative medicine; Tissue engineeringOrganisational unit
03949 - Zenobi-Wong, Marcy / Zenobi-Wong, Marcy
Funding
964497 - ENable LIGHT- and synthetic biology-driven volumetric bioprinting of functional human tissues (EC)
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