Differences in carbon metabolic capacity fuel co-existence and plasmid transfer between Salmonella strains in the mouse gut
Abstract
Antibiotic resistance plasmids can be disseminated between different Enterobacteriaceae in the gut. Here, we investigate how closely related Enterobacteriaceae populations with similar nutrient needs can co-bloom in the same gut and thereby facilitate plasmid transfer. Using different strains of Salmonella Typhimurium (S.Tm SL1344 and ATCC14028) and mouse models of Salmonellosis, we show that the bloom of one strain (i.e., recipient) from very low numbers in a gut pre-occupied by the other strain (i.e., donor) depends on strain-specific utilization of a distinct carbon source, galactitol or arabinose. Galactitol-dependent growth of the recipient S.Tm strain promotes plasmid transfer between non-isogenic strains and between E. coli and S.Tm. In mice stably colonized by a defined microbiota (OligoMM12), galactitol supplementation similarly facilitates co-existence of two S.Tm strains and promotes plasmid transfer. Our work reveals a metabolic strategy used by Enterobacteriaceae to expand in a pre-occupied gut and provides promising therapeutic targets for resistance plasmids spread. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000622475Publication status
publishedExternal links
Journal / series
Cell Host & MicrobeVolume
Pages / Article No.
Publisher
Cell PressSubject
Plasmid transfer; Salmonella; Galactitol; E. coli; Antibiotic resistance; Gut colonization; Cobloom; Arabinose; Microbiota; Colonization resistanceOrganisational unit
03589 - Hardt, Wolf-Dietrich / Hardt, Wolf-Dietrich
Funding
167121 - Towards quantification of the contribution of plasmids to the spread of antibiotic resistance (SNF)
173338 - Deciphering the initial steps that lead to Salmonella Typhimurium diarrhea (SNF)
192567 - Mechanisms controlling the Salmonella Typhimurium gut infection (SNF)
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