Protocol for preparing formalin-fixed paraffin-embedded musculoskeletal tissue samples from mice for spatial transcriptomics
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Date
2024-06-21Type
- Journal Article
ETH Bibliography
yes
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Abstract
Here, we present a protocol for using spatial transcriptomics in bone and multi-tissue musculoskeletal formalin-fixed paraffin-embedded (FFPE) samples from mice. We describe steps for tissue harvesting, sample preparation, paraffin embedding, and FFPE sample selection. We detail procedures for sectioning and placement on spatial slides prior to imaging, decrosslinking, library preparation, and final analyses of the sequencing data. The complete protocol takes ca. 18 days for mouse femora with adjacent muscle; of this time, >50% is required for mineralized tissue decalcification. For complete details on the use and execution of this protocol, please refer to Wehrle et al.1 and Mathavan et al.2 Show more
Permanent link
https://doi.org/10.3929/ethz-b-000668398Publication status
publishedExternal links
Journal / series
STAR ProtocolsVolume
Pages / Article No.
Publisher
Cell PressFunding
741883 - In Vivo Single-Cell Mechanomics of Bone Adaptation and Regeneration in the Aging Mouse (EC)
198152 - Spatial genomics of musculoskeletal regeneration in a translational mouse model of premature aging (SNF)
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ETH Bibliography
yes
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