Light Color-Controlled pH-Adjustment of Aqueous Solutions Using Engineered Proteoliposomes
Open access
Date
2024-04-17Type
- Journal Article
ETH Bibliography
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Abstract
Controlling the pH at the microliter scale can be useful for applications in research, medicine, and industry, and therefore represents a valuable application for synthetic biology and microfluidics. The presented vesicular system translates light of different colors into specific pH changes in the surrounding solution. It works with the two light-driven proton pumps bacteriorhodopsin and blue light-absorbing proteorhodopsin Med12, that are oriented in opposite directions in the lipid membrane. A computer-controlled measuring device implements a feedback loop for automatic adjustment and maintenance of a selected pH value. A pH range spanning more than two units can be established, providing fine temporal and pH resolution. As an application example, a pH-sensitive enzyme reaction is presented where the light color controls the reaction progress. In summary, light color-controlled pH-adjustment using engineered proteoliposomes opens new possibilities to control processes at the microliter scale in different contexts, such as in synthetic biology applications. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000669706Publication status
publishedExternal links
Journal / series
Advanced ScienceVolume
Pages / Article No.
Publisher
Wiley-VCHOrganisational unit
02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.
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ETH Bibliography
yes
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