Show simple item record

dc.contributor.author
Schmidt, Florian I.
dc.contributor.author
Bleck, Christopher K.E.
dc.contributor.author
Reh, Lucia
dc.contributor.author
Novy, Karel
dc.contributor.author
Wollscheid, Bernd
dc.contributor.author
Helenius, Ari
dc.contributor.author
Stahlberg, Henning
dc.contributor.author
Mercer, Jason
dc.date.accessioned
2018-09-25T09:32:08Z
dc.date.available
2017-06-10T19:49:54Z
dc.date.available
2018-09-25T09:32:08Z
dc.date.issued
2013-08
dc.identifier.other
10.1016/j.celrep.2013.06.028
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/70158
dc.identifier.doi
10.3929/ethz-b-000070158
dc.description.abstract
Host cell entry of vaccinia virus, the prototypic poxvirus, involves a membrane fusion event delivering the viral core and two proteinaceous lateral bodies (LBs) into the cytosol. Uncoating of viral cores is poorly characterized, and the composition and function of LBs remains enigmatic. We found that cytosolic cores rapidly dissociated from LBs and expanded in volume, which coincided with reduction of disulfide-bonded core proteins. We identified the abundant phosphoprotein F17, the dual-specificity phosphatase VH1, and the oxidoreductase G4 as bona fide LB components. After reaching the cytosol, F17 was degraded in a proteasome-dependent manner. Proteasome activity, and presumably LB disassembly, was required for the immediate immunomodulatory activity of VH1: dephosphorylation of STAT1 to prevent interferon-γ-mediated antiviral responses. These results reveal a mechanism used by poxviruses to deliver viral enzymes to the host cell cytosol and are likely to facilitate the identification of additional LB-resident viral effectors.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.title
Vaccinia Virus Entry Is Followed by Core Activation and Proteasome-Mediated Release of the Immunomodulatory Effector VH1 from Lateral Bodies
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
ethz.journal.title
Cell Reports
ethz.journal.volume
4
en_US
ethz.journal.issue
3
en_US
ethz.pages.start
464
en_US
ethz.pages.end
476
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
007612702
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
03495 - Helenius, Ari
en_US
ethz.leitzahl.certified
03495 - Helenius, Ari
ethz.date.deposited
2017-06-10T19:51:39Z
ethz.source
ECIT
ethz.identifier.importid
imp593650db322ed57179
ethz.ecitpid
pub:111045
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T09:02:01Z
ethz.rosetta.lastUpdated
2018-09-25T09:32:15Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Vaccinia%20Virus%20Entry%20Is%20Followed%20by%20Core%20Activation%20and%20Proteasome-Mediated%20Release%20of%20the%20Immunomodulatory%20Effector%20VH1%20from%20Lateral%2&rft.jtitle=Cell%20Reports&rft.date=2013-08&rft.volume=4&rft.issue=3&rft.spage=464&rft.epage=476&rft.au=Schmidt,%20Florian%20I.&Bleck,%20Christopher%20K.E.&Reh,%20Lucia&Novy,%20Karel&Wollscheid,%20Bernd&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record