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dc.contributor.author
Faso, Carmen
dc.contributor.author
Bischof, Sylvain
dc.contributor.author
Hehl, Adrian B.
dc.date.accessioned
2018-10-11T07:45:13Z
dc.date.available
2017-06-11T04:52:50Z
dc.date.available
2018-10-11T07:45:13Z
dc.date.issued
2013-12-31
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0083207
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/80452
dc.identifier.doi
10.3929/ethz-b-000080452
dc.description.abstract
Giardia lamblia is an intestinal protozoan parasite required to survive in the environment in order to be transmitted to a new host. To ensure parasite survival, flagellated trophozoites colonizing the small intestine differentiate into non-motile environmentally-resistant cysts which are then shed in the environment. This cell differentiation process called encystation is characterized by significant morphological remodeling which includes secretion of large amounts of cyst wall material. Although much is known about the transcriptional regulation of encystation and the synthesis and trafficking of cyst wall material, the investigation of global changes in protein content and abundance during G. lamblia encystation is still unaddressed. In this study, we report on the quantitative analysis of the G. lamblia proteome during encystation using tandem mass spectrometry. Quantification of more than 1000 proteins revealed major changes in protein abundance in early, mid and late encystation, notably in constitutive secretory protein trafficking. Early stages of encystation were marked by a striking decrease of endoplasmic reticulum-targeted variant-specific surface proteins and significant increases in cytoskeleton regulatory components, NEK protein kinases and proteins involved in protein folding and glycolysis. This was in stark contrast to cells in the later stages of encystation which presented a surprisingly similar proteome composition to non-encysting trophozoites. Altogether these data constitute the first quantitative atlas of the Giardia proteome covering the whole process of encystation and point towards an important role for post-transcriptional control of gene expression in Giardia differentiation. Furthermore, our data provide a valuable resource for the community-based annotation effort of the G. lamblia genome, where almost 70% of all predicted gene models remains “hypothetical”.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
The Proteome Landscape of Giardia lamblia Encystation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e83207
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T04:56:26Z
ethz.source
ECIT
ethz.identifier.importid
imp5936519e71c5023196
ethz.ecitpid
pub:126310
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T23:54:14Z
ethz.rosetta.lastUpdated
2018-10-11T07:45:18Z
ethz.rosetta.versionExported
true
ethz.COinS
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