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dc.contributor.author
Gupta, Sandeep
dc.contributor.author
Gach, Johannes S.
dc.contributor.author
Becerra, Juan C.
dc.contributor.author
Phan, Tran B.
dc.contributor.author
Pudney, Jeffrey
dc.contributor.author
Moldoveanu, Zina
dc.contributor.author
Joseph, Sarah B.
dc.contributor.author
Landucci, Gary
dc.contributor.author
Supnet, Medalyn J.
dc.contributor.author
Ping, Li-Hua
dc.contributor.author
Corti, Davide
dc.contributor.author
Moldt, Brian
dc.contributor.author
Hel, Zdenek
dc.contributor.author
Lanzavecchia, Antonio
dc.contributor.author
Ruprecht, Ruth M.
dc.contributor.author
Burton, Dennis R.
dc.contributor.author
Mestecky, Jiri
dc.contributor.author
Anderson, Deborah J.
dc.contributor.author
Forthal, Donald N.
dc.date.accessioned
2018-11-27T11:08:56Z
dc.date.available
2017-06-11T05:14:32Z
dc.date.available
2018-11-06T15:24:44Z
dc.date.available
2018-11-27T11:08:56Z
dc.date.issued
2013-11-21
dc.identifier.other
10.1371/journal.ppat.1003776
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/80795
dc.identifier.doi
10.3929/ethz-b-000080795
dc.description.abstract
The mechanisms by which human immunodeficiency virus type 1 (HIV-1) crosses mucosal surfaces to establish infection are unknown. Acidic genital secretions of HIV-1-infected women contain HIV-1 likely coated by antibody. We found that the combination of acidic pH and Env-specific IgG, including that from cervicovaginal and seminal fluids of HIV-1-infected individuals, augmented transcytosis across epithelial cells as much as 20-fold compared with Env-specific IgG at neutral pH or non-specific IgG at either pH. Enhanced transcytosis was observed with clinical HIV-1 isolates, including transmitted/founder strains, and was eliminated in Fc neonatal receptor (FcRn)-knockdown epithelial cells. Non-neutralizing antibodies allowed similar or less transcytosis than neutralizing antibodies. However, the ratio of total:infectious virus was higher for neutralizing antibodies, indicating that they allowed transcytosis while blocking infectivity of transcytosed virus. Immunocytochemistry revealed abundant FcRn expression in columnar epithelia lining the human endocervix and penile urethra. Acidity and Env-specific IgG enhance transcytosis of virus across epithelial cells via FcRn and could facilitate translocation of virus to susceptible target cells following sexual exposure.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
The Neonatal Fc Receptor (FcRn) Enhances Human Immunodeficiency Virus Type 1 (HIV-1) Transcytosis across Epithelial Cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS pathogens
ethz.journal.volume
9
en_US
ethz.journal.issue
11
en_US
ethz.pages.start
e1003776
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
005409548
ethz.publication.place
Lawrence, KS
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T05:15:36Z
ethz.source
ECIT
ethz.identifier.importid
imp593651a60cc7641939
ethz.ecitpid
pub:126919
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T13:55:08Z
ethz.rosetta.lastUpdated
2019-01-03T12:32:06Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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