The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis

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Date
2014-03-21Type
- Journal Article
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Cited 28 times in
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Cited 29 times in
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Abstract
Vitamin B6 is an indispensable compound for survival, well known as a cofactor for numerous central metabolic enzymes and more recently for playing a role in several stress responses, particularly in association with oxidative stress. Regulatory aspects for the use of the vitamin in these roles are not known. Here we show that certain plants carry a pseudoenzyme (PDX1.2), which is involved in regulating vitamin B6 biosynthesis de novo under stress conditions. Specifically, we demonstrate that Arabidopsis PDX1.2 enhances the activity of its catalytic paralogs by forming a heterododecameric complex. PDX1.2 is strongly induced by heat as well as singlet oxygen stress, concomitant with an enhancement of vitamin B6 production. Analysis of pdx1.2 knockdown lines demonstrates that boosting vitamin B6 content is dependent on PDX1.2, revealing that this pseudoenzyme acts as a positive regulator of vitamin B6 biosynthesis during such stress conditions in plants. Show more
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https://doi.org/10.3929/ethz-b-000081902Publication status
publishedExternal links
Journal / series
Journal of Biological ChemistryVolume
Pages / Article No.
Publisher
American Society for Biochemistry and Molecular BiologySubject
Antioxidants; Biosynthesis; Enzyme Catalysis; Plant Biochemistry; Pyridoxal Phosphate; Stress ResponseMore
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Citations
Cited 28 times in
Web of Science
Cited 29 times in
Scopus
ETH Bibliography
yes
Altmetrics