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dc.contributor.author
Rendueles, Olaya
dc.contributor.author
Travier, Laetitia
dc.contributor.author
Latour-Lambert, Patricia
dc.contributor.author
Fontaine, Thierry
dc.contributor.author
Magnus, Julle
dc.contributor.author
Denamur, Erick
dc.contributor.author
Ghigo, Jean-Marc
dc.date.accessioned
2019-09-03T10:39:07Z
dc.date.available
2017-06-11T10:18:26Z
dc.date.available
2019-09-03T10:39:07Z
dc.date.issued
2011
dc.identifier.issn
2150-7511
dc.identifier.other
10.1128/mBio.00043-11
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/86059
dc.identifier.doi
10.3929/ethz-b-000086059
dc.description.abstract
Bacterial biofilms often form multispecies communities in which complex but ill-understood competition and coop-eration interactions occur. In light of the profound physiological modifications associated with this lifestyle, we hypothesizedthat the biofilm environment might represent an untapped source of natural bioactive molecules interfering with bacterial adhe-sion or biofilm formation. We produced cell-free solutions extracted fromin vitromature biofilms formed by 122 naturalEsche-richia coliisolates, and we screened these biofilm extracts for antiadhesion molecules active on a panel of Gram-positive andGram-negative bacteria. Using this approach, we showed that 20% of the tested biofilm extracts contained molecules that antag-onize bacterial growth or adhesion. We characterized a compound, produced by a commensal animalE. colistrain, for whichactivity is detected only in biofilm extract. Biochemical and genetic analyses showed that this compound corresponds to a newtype of released high-molecular-weight polysaccharide whose biofilm-associated production is regulated by the RfaH protein.We demonstrated that the antiadhesion activity of this polysaccharide was restricted to Gram-positive bacteria and that its pro-duction reduced susceptibility to invasion and provided rapid exclusion ofStaphylococcus aureusfrom mixedE. coliandS. au-reusbiofilms. Our results therefore demonstrate that biofilms contain molecules that contribute to the dynamics of mixed bacte-rial communities and that are not or only poorly detected in unconcentrated planktonic supernatants. Systematic identificationof these compounds could lead to strategies that limit pathogen surface colonization and reduce the burden associated with thedevelopment of bacterial biofilms on medical devices.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.title
Screening of Escherichia coli species biodiversity reveals new biofilm-associated antiadhesion polysaccharides
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2011-05-10
ethz.journal.title
mBio
ethz.journal.volume
2
en_US
ethz.journal.issue
3
en_US
ethz.pages.start
e00043
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006164914
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03939 - Velicer, Gregory J. / Velicer, Gregory J.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03939 - Velicer, Gregory J. / Velicer, Gregory J.
ethz.date.deposited
2017-06-11T10:19:32Z
ethz.source
ECIT
ethz.identifier.importid
imp5936520cb6b5989697
ethz.ecitpid
pub:135521
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T02:55:55Z
ethz.rosetta.lastUpdated
2019-09-03T10:39:18Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Screening%20of%20Escherichia%20coli%20species%20biodiversity%20reveals%20new%20biofilm-associated%20antiadhesion%20polysaccharides&rft.jtitle=mBio&rft.date=2011&rft.volume=2&rft.issue=3&rft.spage=e00043&rft.issn=2150-7511&rft.au=Rendueles,%20Olaya&Travier,%20Laetitia&Latour-Lambert,%20Patricia&Fontaine,%20Thierry&Magnus,%20Julle&rft.genre=article&
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