Mutation Rate Inferred From Synonymous Substitutions in a Long-Term Evolution Experiment With Escherichia coli
- Journal Article
Rights / licenseCreative Commons Attribution 3.0 Unported
The quantification of spontaneous mutation rates is crucial for a mechanistic understanding of the evolutionary process. In bacteria, traditional estimates using experimental or comparative genetic methods are prone to statistical uncertainty and consequently estimates vary by over one order of magnitude. With the advent of next-generation sequencing, more accurate estimates are now possible. We sequenced 19 Escherichia coli genomes from a 40,000-generation evolution experiment and directly inferred the point-mutation rate based on the accumulation of synonymous substitutions. The resulting estimate was 8.9 × 10−11 per base-pair per generation, and there was a significant bias toward increased AT-content. We also compared our results with published genome sequence datasets for other bacterial evolution experiments. Given the power of our approach, our estimate represents the most accurate measure of bacterial base-substitution rates available to date. Show more
Journal / seriesG3: Genes, Genomes, Genetics
Pages / Article No.
PublisherGenetics Society of America
SubjectGenomic basesubstitution rate; Experimental evolution; Molecular evolution; Mutation pressure; Next-generation sequencing
Organisational unit03939 - Velicer, Gregory J. / Velicer, Gregory J.
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