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An integrated workflow for charting the human interaction proteome: insights into the PP2A system
(2009)Molecular Systems BiologyProtein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP‐MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP‐MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its ...Journal Article -
Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly
(2009)The Journal of Cell BiologyIn animal somatic cells, bipolar spindle formation requires separation of the centrosome-based spindle poles. Centrosome separation relies on multiple pathways, including cortical forces and antiparallel microtubule (MT) sliding, which are two activities controlled by the protein kinase aurora A. We previously found that depletion of the human kinetochore protein Mcm21RCENP-O results in monopolar spindles, raising the question as to whether ...Journal Article -
Htm1 protein generates the N-glycan signal for glycoprotein degradation in the endoplasmic reticulum
(2009)The Journal of Cell BiologyTo maintain protein homeostasis in secretory compartments, eukaryotic cells harbor a quality control system that monitors protein folding and protein complex assembly in the endoplasmic reticulum (ER). Proteins that do not fold properly or integrate into cognate complexes are degraded by ER-associated degradation (ERAD) involving retrotranslocation to the cytoplasm and proteasomal peptide hydrolysis. N-linked glycans are essential in ...Journal Article -
Sem1 is a functional component of the nuclear pore complex–associated messenger RNA export machinery
(2009)The Journal of Cell BiologyThe evolutionarily conserved protein Sem1/Dss1 is a subunit of the regulatory particle (RP) of the proteasome, and, in mammalian cells, binds the tumor suppressor protein BRCA2. Here, we describe a new function for yeast Sem1. We show that sem1 mutants are impaired in messenger RNA (mRNA) export and transcription elongation, and induce strong transcription-associated hyper-recombination phenotypes. Importantly, Sem1, independent of the ...Journal Article -
Distinct cytoplasmic maturation steps of 40S ribosomal subunit precursors require hRio2
(2009)The Journal of Cell BiologyDuring their biogenesis, 40S ribosomal subunit precursors are exported from the nucleus to the cytoplasm, where final maturation occurs. In this study, we show that the protein kinase human Rio2 (hRio2) is part of a late 40S preribosomal particle in human cells. Using a novel 40S biogenesis and export assay, we analyzed the contribution of hRio2 to late 40S maturation. Although hRio2 is not absolutely required for pre-40S export, deletion ...Journal Article -
FOXC2 controls formation and maturation of lymphatic collecting vessels through cooperation with NFATc1
(2009)The Journal of Cell BiologyThe mechanisms of blood vessel maturation into distinct parts of the blood vasculature such as arteries, veins, and capillaries have been the subject of intense investigation over recent years. In contrast, our knowledge of lymphatic vessel maturation is still fragmentary. In this study, we provide a molecular and morphological characterization of the major steps in the maturation of the primary lymphatic capillary plexus into collecting ...Journal Article -
Integrin-linked kinase is required for radial sorting of axons and Schwann cell remyelination in the peripheral nervous system
(2009)The Journal of Cell BiologyDuring development, Schwann cells (SCs) interpret different extracellular cues to regulate their migration, proliferation, and the remarkable morphological changes associated with the sorting, ensheathment, and myelination of axons. Although interactions between extracellular matrix proteins and integrins are critical to some of these processes, the downstream signaling pathways they control are still poorly understood. Integrin-linked ...Journal Article -
Bub1 regulates chromosome segregation in a kinetochore-independent manner
(2009)The Journal of Cell BiologyThe kinetochore-bound protein kinase Bub1 performs two crucial functions during mitosis: it is essential for spindle checkpoint signaling and for correct chromosome alignment. Interestingly, Bub1 mutations are found in cancer tissues and cancer cell lines. Using an isogenic RNA interference complementation system in transformed HeLa cells and untransformed RPE1 cells, we investigate the effect of structural Bub1 mutants on chromosome ...Journal Article -
Sensor for Measuring Strain in Textile
(2008)SensorsIn this paper a stain sensor to measure large strain (80%) in textiles is presented. It consists of a mixture of 50wt-% thermoplastic elastomer (TPE) and 50wt-% carbon black particles and is fiber-shaped with a diameter of 0.315mm. The attachment of the sensor to the textile is realized using a silicone film. This sensor configuration was characterized using a strain tester and measuring the resistance (extension-retraction cycles): It ...Journal Article -
Alpine grassland phenology as seen in AVHRR, VEGETATION, and MODIS NDVI time series - a comparison with in situ measurements
(2008)SensorsThis study evaluates the ability to track grassland growth phenology in the Swiss Alps with NOAA-16 Advanced Very High Resolution Radiometer (AVHRR) Normalized Difference Vegetation Index (NDVI) time series. Three growth parameters from 15 alpine and subalpine grassland sites were investigated between 2001 and 2005: Melt-Out (MO), Start Of Growth (SOG), and End Of Growth (EOG).We tried to estimate these phenological dates from yearly NDVI ...Journal Article