Search
Results
-
PhenoChip: A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae
(2020)Science AdvancesPhotosynthetic microorganisms are key players in aquatic ecosystems with strong potential for bioenergy production, yet their systematic selection at the single-cell level for improved productivity or stress resilience (“phenotyping”) has remained largely inaccessible. To facilitate the phenotyping of microalgae and cyanobacteria, we developed “PhenoChip,” a platform for the multiparametric photophysiological characterization and selection ...Journal Article -
Comprehensive characterization of myeloid cells during wound healing in healthy and healing‐impaired diabetic mice
(2020)European Journal of ImmunologyWound healing involves the concerted action of various lymphoid and in particular myeloid cell populations. To characterize and quantitate different types of myeloid cells and to obtain information on their kinetics during wound healing, we performed multiparametric flow cytometry analysis. In healthy mice, neutrophil numbers increased early after injury and returned to near basal levels after completion of healing. Macrophages, ...Journal Article -
Cortical Excitation:Inhibition Imbalance Causes Abnormal Brain Network Dynamics as Observed in Neurodevelopmental Disorders
(2020)Cerebral CortexAbnormal brain development manifests itself at different spatial scales. However, whether abnormalities at the cellular level can be diagnosed from network activity measured with functional magnetic resonance imaging (fMRI) is largely unknown, yet of high clinical relevance. Here a putative mechanism reported in neurodevelopmental disorders, that is, excitation-to-inhibition ratio (E:I), was chemogenetically increased within cortical ...Journal Article -
What drives public support for policies to enhance electric vehicle adoption?
(2020)Environmental Research LettersDecarbonizing the transportation sector is crucial to limiting global warming, but faces severe political feasibility challenges due to widespread opposition by those who incur the costs. With respect to private motorized vehicles, which account for the largest share of emissions from transportation, various studies show that pull measures, such as subsidies for electric cars (EVs) and charging infrastructure, attract more public support ...Journal Article -
Single-molecule and Single-cell Approaches in Molecular Bioengineering
(2020)ChimiaProtein sequences inhabit a discrete set in macromolecular space with incredible capacity to treat human disease. Despite our ability to program and manipulate protein sequences, the vast majority of protein development efforts are still done heuristically without a unified set of guiding principles. This article highlights work in understanding biophysical stability and function of proteins, developing new biophysical measurement tools ...Journal Article -
One by One - Insights into Complex Immune Responses through Functional Single-cell Analysis
(2020)ChimiaImmune responses are highly dynamic and complex. The successful completion thereof involves and needs many different cells from the immune system, and requires their specific interactions and functions. Individual cells are the functional units within any immune response, and their varying frequencies and degrees of activity shape and define the response. The state, activation and ultimately functionality of immune cells displays high ...Journal Article -
New Catalysis Concepts for Molecular Design and Feedstocks Valorization
(2020)ChimiaThe discovery and preparation of important molecules rely on the synthetic chemist's ability to valorize common feedstock materials, such as petroleum or biomass. Key to this endeavor is the design of efficient methods to introduce, remove or interconvert functional groups. Our group has a longstanding interest in developing conceptually novel catalytic reactions for the installation of unprotected amines, the selective activation of C-O ...Journal Article -
Reprogrammable shape morphing of magnetic soft machines
(2020)Science AdvancesShape-morphing magnetic soft machines are highly desirable for diverse applications in minimally invasive medicine, wearable devices, and soft robotics. Despite recent progress, current magnetic programming approaches are inherently coupled to sequential fabrication processes, preventing reprogrammability and high-throughput programming. Here, we report a high-throughput magnetic programming strategy based on heating magnetic soft materials ...Journal Article -
A universal method for the rapid isolation of all known classes of functional silencing small RNAs
(2020)Nucleic Acids ResearchDiverse classes of silencing small (s)RNAs operate via ARGONAUTE-family proteins within RNA-induced-silencing-complexes (RISCs). Here, we have streamlined various embodiments of a Q-sepharose-based RISC-purification method that relies on conserved biochemical properties of all ARGONAUTEs. We show, in multiple benchmarking assays, that the resulting 15-min benchtop extraction procedure allows simultaneous purification of all known classes ...Journal Article -
Eukaryotic life without tQ(CUG): the role of Elongator-dependent tRNA modifications in Dictyostelium discoideum
(2020)Nucleic Acids ResearchIn the Elongator-dependent modification pathway, chemical modifications are introduced at the wobble uridines at position 34 in transfer RNAs (tRNAs), which serve to optimize codon translation rates. Here, we show that this three-step modification pathway exists in Dictyostelium discoideum, model of the evolutionary superfamily Amoebozoa. Not only are previously established modifications observable by mass spectrometry in strains with the ...Journal Article