Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics
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Date
2022-03-18Type
- Journal Article
ETH Bibliography
yes
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Abstract
Due to the low stoichiometry and highly transient nature of protein phosphorylation it is challenging to capture the dynamics and complexity of phosphorylation events on a systems level. Here, we present an optimized protocol to measure virus-induced phosphorylation events with high sensitivity using label free quantification-based phosphoproteomics. Specifically, we describe filter assisted protein digestion (FASP), enrichment of phosphopeptides, mass spectrometry, and subsequent bioinformatic analysis. For complete details on the use and execution of this protocol, please refer to Hunziker et al. (2022). Show more
Permanent link
https://doi.org/10.3929/ethz-b-000527717Publication status
publishedExternal links
Journal / series
STAR ProtocolsVolume
Pages / Article No.
Publisher
Cell PressMore
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ETH Bibliography
yes
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